LOCUS       Exported               11601 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pGA643, complete sequence.
ACCESSION   AY804024
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   An G, Ebert PR, Mitra A, Ha SB.
  TITLE     Binary Vectors
  JOURNAL   (in) Gelvin,S.B. and Schilperoort,R.A. (Eds.); PLANT MOLECULAR 
            BIOLOGY MANUAL: A3/1-19; Kluwer Academic Publishers (1988)
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   Kim S-R., Kang H-G., Lee S, Kim K-M., An G.
  TITLE     Complete sequence of the pGA643 binary vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11601)
  AUTHORS   Kim S-R., Kang H-G., Lee S, Kim K-M., An G.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2004) Division of Molecular and Life Science, 
            POSTECH, Nam-Gu Hyoja San31, Pohang, Kyungbuk 790-784, Republic of 
            Korea
REFERENCE   4  (bases 1 to 11601)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Binary vector pGA643"
                     /mol_type="other DNA"
                     /db_xref="taxon:301028"
     misc_feature    1..25
                     /label=right border of Ti-plasmid
                     /note="right border of Ti-plasmid"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      160..364
                     /regulatory_class="promoter"
                     /note="promoter of the nos gene"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             365..1186
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="neomycin phosphotransferase II"
                     /label=nos-nptII
                     /note="kanamycin resistance protein"
                     /protein_id="AAV66537.1"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            365..1186
                     /gene="nos-nptII"
                     /label=nos-nptII
     terminator      1811..2063
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      1811..2062
                     /regulatory_class="terminator"
                     /note="3UTR of the nos gene"
     regulatory      2397..2812
                     /regulatory_class="promoter"
                     /note="35S promoter of CaMV"
     promoter        2471..2815
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2824..2862
                     /label=polylinker
                     /note="polylinker"
     regulatory      2864..3075
                     /regulatory_class="terminator"
                     /note="terminator sequence of T-DNA gene 7"
     terminator      2875..3075
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    complement(3076..3601)
                     /label=3UTR of T-DNA gene 5
                     /note="3UTR of T-DNA gene 5"
     misc_feature    3678..3702
                     /label=left border of Ti-plasmid
                     /note="left border of Ti-plasmid"
     oriT            4257..4366
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     CDS             4399..4770
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /protein_id="AAV66538.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     gene            4399..4770
                     /gene="traJ"
                     /label=traJ
     CDS             5042..6190
                     /codon_start=1
                     /gene="trfA"
                     /product="TrfA"
                     /label=trfA
                     /note="trans-acting replication"
                     /protein_id="AAV66539.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            5042..6190
                     /gene="trfA"
                     /label=trfA
     CDS             complement(7463..8662)
                     /codon_start=1
                     /gene="tetA"
                     /product="TetA"
                     /label=tetA
                     /note="tetracycline resistance"
                     /protein_id="AAV66540.1"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     gene            complement(7463..8662)
                     /gene="tetA"
                     /label=tetA
     CDS             8768..9418
                     /codon_start=1
                     /gene="tetR"
                     /product="TetR"
                     /label=tetR
                     /note="tetracycline resistance regulation"
                     /protein_id="AAV66541.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            8768..9418
                     /gene="tetR"
                     /label=tetR
     rep_origin      10413..11121
                     /note="OriV; incP origin of replication"
     rep_origin      10483..11114
                     /label=oriV
                     /note="incP origin of replication"