LOCUS Exported 8742 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Binary vector pCAMBIA-2300, complete sequence.
ACCESSION AF234315
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8742)
AUTHORS Hajdukiewicz P, Svab Z, Maliga P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors for
plant transformation
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
PUBMED 7919218
REFERENCE 2 (bases 1 to 8742)
AUTHORS Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet
I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A,
Jefferson RA.
TITLE A comprehensive set of modular vectors for advanced manipulations
and efficient transformation of plants
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8742)
AUTHORS Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet
I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A,
Jefferson RA.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
GPO Box 3200, Canberra, ACT 2601, Australia
REFERENCE 4 (bases 1 to 8742)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8742
/organism="Binary vector pCAMBIA-2300"
/mol_type="other DNA"
/db_xref="taxon:118407"
misc_feature complement(980..1980)
/label=STA region from pVS1 plasmid
/note="STA region from pVS1 plasmid"
CDS 1219..1848
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2282..3349
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin complement(2573..3573)
/direction=LEFT
/note="pVS1-REP; replication origin from pVS1"
rep_origin 3415..3609
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 3953..4093
/label=bom
/note="basis of mobility region from pBR322"
misc_feature complement(3983..4243)
/label=bom site from pBR322
/note="bom site from pBR322"
rep_origin complement(4279..4867)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin complement(4383..4663)
/direction=LEFT
/label=pBR322 origin of replication
/note="pBR322 origin of replication"
CDS complement(4954..5748)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=aminoglycoside phosphotransferase
/note="aadA (kanamycin resistance) gene amplified from
pIG121Hm"
/protein_id="AAF65399.1"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6173..6198
/label=left border repeat from C58 T-DNA
/note="left border repeat from C58 T-DNA"
misc_feature 6173..6197
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
misc_feature 6265..6468
/label=CaMV 3UTR (polyA signal)
/note="CaMV 3UTR (polyA signal)"
polyA_signal 6275..6449
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6506..7303)
/codon_start=1
/product="neomycin phosphotransferase"
/label=neomycin phosphotransferase
/note="nptII (kanamycin resistance) gene"
/protein_id="AAF65400.1"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(7334..8114)
/regulatory_class="promoter"
/note="CaMV35S2; CaMV 35S promoter, duplicated"
promoter complement(7366..8043)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 8234..8255
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
regulatory 8262..8316
/regulatory_class="promoter"
/note="PlacZ; lacZ promoter"
promoter 8270..8300
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8308..8324
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8332..8348
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 8344..8577
/codon_start=1
/product="LacZ alpha fragment"
/label=LacZ alpha fragment
/protein_id="AAF65398.1"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 8358..8414
/note="pUC18 MCS; polylinker"
primer_bind complement(8418..8434)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 8637..8661
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
misc_feature 8656..8681
/label=right border T-DNA repeat
/note="right border T-DNA repeat"
