LOCUS       Exported                4886 bp ds-DNA    circular SYN 07-1-2013
DEFINITION  Plasmid for expressing low levels of T7 lysozyme, an inhibitor of T7
            RNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    pLysS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
COMMENT     The T7 lysozyme gene is not highly expressed because it is in the 
            antisense orientation relative to the tet promoter.
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        complement(220..322)
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
     rep_origin      complement(848..1392)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1504..1532
                     /note="tet promoter"
                     /note="promoter for tetracycline efflux protein gene"
     promoter        complement(1981..2014)
                     /note="T7 Phi-3.8 promoter"
                     /note="weak promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2012..2467)
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /note="T7 lysozyme"
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAGLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG"
     CDS             complement(4446..219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"