LOCUS       Exported                3991 bp ds-DNA    circular SYN 30-1-2014
DEFINITION  Mammalian vector encoding a promoter that can be activated by 
            drug-induced heterodimerization of ZFHD1 and the p65 activation 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    pZFHD1-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     protein_bind    88..297
                     /label="12  ZFHD1 binding sites"
                     /bound_moiety="ZFHD1"
                     /note="ZFHD1 binding sites"
                     /note="12 binding sites for the composite human DNA-binding
                     domain ZFHD1 (Pomerantz et al., 1995)"
     promoter        320..433
                     /label="IL-2 promoter"
                     /note="minimal IL-2 promoter"
                     /note="minimal promoter from the human interleukin-2 gene"
     misc_feature    462..505
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    619..740
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(747..1202)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1229..1333
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        1335..1692
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1543..1678
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             1727..2521
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2753..2800
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      3129..3717
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3779..3827
                     /note="synthetic polyadenylation signal"
     misc_feature    3841..3932
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"