| 载体基本信息 |
| 载体名称 |
pTK-neo |
载体抗性 |
Kanamycin |
| 载体长度 |
2872 bp |
载体类型 |
Mammalian Expression Vectors |
| 载体来源 |
Novagen (EMD Millipore) |
筛选标记 |
Neomycin/G418(Geneticin) |
| 拷贝数 |
High copy number |
pTK-neo 载体载体图谱
pTK-neo 载体载体序列
LOCUS Exported 2872 bp ds-DNA circular SYN 21-1-2014
DEFINITION Mammalian vector encoding a neomycin resistance gene, to select for
stable integration of a cotransfected expression plasmid.
ACCESSION .
VERSION .
KEYWORDS pTK-neo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2872)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..2872
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
CDS 816..1610
/codon_start=1
/gene="aph(3)-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 1842..1889
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 2218..2806
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2870..749
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
