LOCUS       Exported                7002 bp ds-DNA    circular SYN 31-1-2014
DEFINITION  Mammalian vector encoding four FKBP homodimerizer domains, for 
            creating ER-localized oligomers that can be dissociated with a drug.
            Also known as pC4S1-FM4-FCS-hGH.
ACCESSION   .
VERSION     .
KEYWORDS    prHom-Sec1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     Clone between the SpeI and BamHI sites to replace the stuffer 
            sequence with the coding sequence of interest. The insert should 
            encode the furin cleavage site and a stop codon.
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        19..322
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5UTR           616..655
                     /note="HSV TK 5-UTR"
                     /note="5 untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             692..769
                     /codon_start=1
                     /product="human growth hormone signal sequence"
                     /label="Signal sequence"
                     /note="hGH signal sequence"
                     /translation="MATGSRTSLLLAFGLLCLPWLQEGSA"
     CDS             776..1096
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /note="DmrD"
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1103..1423
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /note="DmrD"
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1430..1750
                     /codon_start=1
                     /gene="
                     "
                     /gene_synonym="
                     "
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /EC_number="
                     "
                     /note="DmrD"
                     /note="dimerization can be reversed by ligand binding"
                     /protein_id="
                     "
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1757..2077
                     /codon_start=1
                     /gene="
                     "
                     /gene_synonym="
                     "
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /EC_number="
                     "
                     /note="DmrD"
                     /note="dimerization can be reversed by ligand binding"
                     /protein_id="
                     "
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2087..2104
                     /codon_start=1
                     /product="cleavage site for the furin protease in the 
                     trans-Golgi network"
                     /note="furin cleavage site"
                     /translation="RNRQKR"
     CDS             2105..2677
                     /codon_start=1
                     /product="
                     "
                     /label="Stuffer sequence"
                     /note="stuffer sequence"
                     /note="to be replaced by the coding sequence of interest"
                     /translation="FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFL
                     QNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYG
                     ASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLL
                     YCFRKDMDKVETFLRIVQCRSVEGSCGF"
     intron          2704..3276
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     polyA_site      3473..3528
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(3861..4056)
                     /label="SV40 promoter"
                     /note="SV40 promoter"
     rep_origin      3881..4016
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(4293..4881)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5052..5924)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(5925..6029)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(6361..6816)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"