LOCUS       Exported                5698 bp ds-DNA    circular SYN 31-1-2014
DEFINITION  Mammalian vector encoding two FKBP homodimerizer domains, for 
            creating cytoplasmic oligomers that can be dissociated with a drug. 
            Also known as pC4EN-FM2E.
ACCESSION   .
VERSION     .
KEYWORDS    prHom-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     Clone into the XbaI site to fuse two copies of DmrD to the 
            C-terminus of the partner protein.
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        19..322
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5UTR           616..655
                     /note="HSV TK 5-UTR"
                     /note="5 untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             676..678
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             688..1008
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /note="DmrD"
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1015..1335
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /note="DmrD"
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1342..1368
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     intron          1400..1972
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2169..2224
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2557..2752)
                     /label="SV40 promoter"
                     /note="SV40 promoter"
     rep_origin      2577..2712
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(2989..3577)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3748..4620)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4621..4725)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(5057..5512)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"