LOCUS       Exported                5726 bp ds-DNA    circular SYN 28-1-2014
DEFINITION  Mammalian vector encoding FKBP homodimerizer domains, for creating 
            membrane-associated fusion proteins that can be dimerized with a 
            drug. Also known as pC4M-FV2E.
ACCESSION   .
VERSION     .
KEYWORDS    pHom-Mem1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     Clone into the XbaI site to fuse the DmrB domains to the C-terminus 
            of the partner protein, or into the SpeI site to fuse the DmrB 
            domains to the N-terminus of the partner protein.
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        19..322
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5UTR           616..655
                     /note="HSV TK 5-UTR"
                     /note="5 untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             668..709
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase"
                     /note="N-myristoylation signal"
                     /translation="MGSSKSKPKDPSQR"
     CDS             716..1036
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1043..1363
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1370..1396
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     intron          1428..2000
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2197..2252
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2585..2780)
                     /label="SV40 promoter"
                     /note="SV40 promoter"
     rep_origin      2605..2740
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(3017..3605)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3776..4648)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4649..4753)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(5085..5540)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"