LOCUS       Exported                8202 bp ds-DNA    circular SYN 30-1-2014
DEFINITION  Mammalian vector encoding a transcription factor activation domain 
            and a DNA-binding domain that can be assembled by drug-induced 
            heterodimerization.
ACCESSION   .
VERSION     .
KEYWORDS    pHet-Act1-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        138..517
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          890..1022
                     /note="chimeric intron"
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             1102..1104
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             1114..1392
                     /codon_start=1
                     /gene="
                     "
                     /gene_synonym="
                     "
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /note="FRB* (DmrC)"
                     /note="binds synthetic ligands such as AP21967"
                     /protein_id="
                     "
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             1399..1971
                     /codon_start=1
                     /product="activation domain from the NF-kappa-B 
                     transcription factor p65"
                     /note="p65 activation domain"
                     /translation="DEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQ
                     APAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFT
                     DLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGL
                     PNGLLSGDEDFSSIADMDFSALLSQISS"
     misc_feature    2040..2613
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2647..2649
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             2656..2682
                     /codon_start=1
                     /product="nuclear localization signal of human c-myc (Dang 
                     and Lee, 1988)"
                     /note="c-myc NLS"
                     /translation="PAAKRVKLD"
     CDS             2689..3051
                     /codon_start=1
                     /product="composite human DNA-binding domain composed of 
                     domains from the transcription factors Zif268 and Oct-1 
                     (Pomerantz et al., 1995)"
                     /label="ZFHD1 (DNA binding domain)"
                     /note="ZFHD1"
                     /translation="ERPYACPVESCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSR
                     SDHLTTHIRTHTGGGRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKE
                     VIRVWFCNRRQKEKRIN"
     CDS             3058..3378
                     /codon_start=1
                     /gene="
                     "
                     /gene_synonym="
                     "
                     /product="human FK506-binding protein FKBP12"
                     /EC_number="
                     "
                     /note="FKBP (DmrA)"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3388..3705
                     /codon_start=1
                     /gene="
                     "
                     /gene_synonym="
                     "
                     /product="human FK506-binding protein FKBP12"
                     /EC_number="
                     "
                     /note="FKBP (DmrA)"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="VQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPF
                     KFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLK
                     LE"
     CDS             3712..4032
                     /codon_start=1
                     /gene="
                     "
                     /gene_synonym="
                     "
                     /product="human FK506-binding protein FKBP12"
                     /EC_number="
                     "
                     /note="FKBP (DmrA)"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    4101..4222
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4408..4863
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4980..5337
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      5188..5323
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             5388..5987
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    6051..6099
                     /note="synthetic polyadenylation signal"
     promoter        6405..6509
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6510..7370
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="Amp R"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7541..8129
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"