LOCUS       Exported                4278 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCMV-Script, complete sequence.
ACCESSION   AF028239
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Hosfield T, Padgett K, Sanchez T, Lu Q.
  TITLE     Mammalian expression vector for efficient cloning of PCR fragments
  JOURNAL   Strategies 10, 68-69 (1997)
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Hosfield T, Padgett K, Sanchez T, Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-1997) Marketing Analysis, Stratagene, 11011 North 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 4278)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Mammalian expression vector pCMV-Script"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:69046"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(780..798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1072..1193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1200..1655)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1682..1786
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1788..2145
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1996..2131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2180..2974
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3206..3253
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3582..4170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"