LOCUS       Exported                3791 bp ds-DNA    circular SYN 07-3-2016
DEFINITION  Vector for expressing an N-terminally Myc-tagged protein in 
            mammalian cells. pCMV-Myc-N is identical but contains a complete Myc
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     This plasmid contains a truncated Myc tag.
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        27..330
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          672..768
                     /note="SV40 intron"
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             829..831
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             841..867
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc (truncated)"
                     /note="The first amino acid of the standard Myc tag is 
                     absent."
                     /translation="QKLISEEDL"
     misc_feature    872..926
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    930..1064
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1175..1191)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1577..2165)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2336..3196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3197..3301)
                     /gene="bla"
                     /note="AmpR promoter"