LOCUS       Exported                3794 bp ds-DNA    circular SYN 07-3-2016
DEFINITION  Mammalian vector for CMV promoter-driven expression of an ORF with 
            an N-terminal Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Myc-N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   transOMIC, Clontech
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        27..330
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          672..768
                     /note="SV40 intron"
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             838..840
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             841..870
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    875..929
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    933..1067
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1178..1194)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1580..2168)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2339..3199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3200..3304)
                     /gene="bla"
                     /note="AmpR promoter"