LOCUS       Exported                7435 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Plasmid for expression of gene of interest in Lactobacillus 
            plantarum and L. sakei.
ACCESSION   .
VERSION     .
KEYWORDS    pSIP403
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Sorvig E, Mathiesen G, Naterstad K, Eijsink VG, Axelsson L
  TITLE     High-level, inducible gene expression in Lactobacillus sakei and 
            Lactobacillus plantarum using versatile expression vectors.
  JOURNAL   Microbiology. 2005 Jul;151(Pt 7):2439-49. doi: 
            10.1099/mic.0.28084-0.
  PUBMED    16000734
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2005-07"; volume: "151(Pt 7):2439-49. doi"; 
            pages: " 10.1099/mic.0.28084-0"
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             575..2383
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQGGKQ"
     rep_origin      2731..3319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3220..3239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"