LOCUS       Exported                4518 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid vector pBK-CMV, complete sequence.
ACCESSION   U37573
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Marsh S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-1995) Marketing Analysis, Stratagene, 11011 North 
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Phagemid vector pBK-CMV"
                     /mol_type="other DNA"
                     /label=ZapExpress vector system
                     /note="ZapExpress vector system"
                     /db_xref="taxon:390862"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      24..330
                     /label=f1
                     /note="f1"
     misc_feature    469..750
                     /label=SV40 polyA
                     /note="SV40 polyA"
     polyA_signal    469..590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(797..1183)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTLITPSSKLTLTKGNKSWSSRACRSTLVDPKNSKSFSRVLLERP
                     RAHRFSTRVGYQVSVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEARTDRPSQQLRSLNGEWRSNF"
     misc_feature    complement(812..1183)
                     /label=beta-galactosidase alpha fragment
                     /note="beta-galactosidase alpha fragment"
     primer_bind     952..968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        975..993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1015..1122
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(1140..1158)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1179..1195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(1184..1305)
                     /regulatory_class="promoter"
                     /note="lac"
     protein_bind    1203..1219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(1306..1895)
                     /regulatory_class="promoter"
                     /note="CMV"
     promoter        complement(1328..1531)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1532..1835
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      1954..2621
                     /label=pUC
                     /note="pUC"
     rep_origin      complement(2010..2598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    2927..2974
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3206..4000)
                     /codon_start=1
                     /product="neomycin/kanamycin resistance protein"
                     /label=neomycin/kanamycin resistance protein
                     /protein_id="ABG26757.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4035..4392)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      complement(4035..4373)
                     /regulatory_class="promoter"
                     /note="SV40"
     rep_origin      4049..4184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      complement(4392..4518)
                     /regulatory_class="promoter"
                     /note="bla"
     promoter        complement(4394..4498)
                     /gene="bla"
                     /label=AmpR promoter