LOCUS       Exported                3740 bp ds-DNA    circular SYN 25-11-2013
DEFINITION  Broad host range expression vector with a kanamycin resistance 
            marker and with the pBBR1 replicon for Gram-negative bacteria.
ACCESSION   DQ058730
VERSION     .
KEYWORDS    pRANGER-BTB-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Lynch MD, Gill RT.
  TITLE     Broad host range vectors for stable genomic library construction.
  JOURNAL   Biotechnol. Bioeng. 2006;94:151-8.
  PUBMED    16496398
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Lucigen
  TITLE     Direct Submission
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="synthetic DNA construct"
                     /lab_host="Gram-negative bacteria"
                     /mol_type="other DNA"
     terminator      60..96
                     /note="soxR terminator"
                     /note="bidirectional E. coli soxR transcription terminator"
     CDS             872..1534
                     /codon_start=1
                     /gene="
                     "
                     /product="replication protein from the broad host range 
                     plasmid pBBR1"
                     /note="pBBR1 Rep"
                     /note="
                     "
                     /experiment="
                     "
                     /protein_id="
                     "
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             1631..2446
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      2463..2494
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2522..3400)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /note="araC"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3546..3711
                     /gene="araBAD"
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction"
     misc_feature    3732..9
                     /note="MCS"
                     /note="multiple cloning site"