LOCUS       Exported                2008 bp ds-DNA    circular SYN 25-11-2013
DEFINITION  Blue/white screening vector with a kanamycin resistance marker for 
            high efficiency blunt end cloning.
ACCESSION   AF532108
VERSION     .
KEYWORDS    pEZSeq-Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Lucigen
  TITLE     Direct Submission
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        18..48
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    56..72
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             92..289
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /protein_id="
                     "
                     /translation="MTMITPSLSTNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWR"
     terminator      324..355
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        356..458
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
     CDS             459..1274
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1295..1322
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1334..1921)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1943..1972
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"