LOCUS       Exported                4103 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSP-luc+NF, complete sequence.
ACCESSION   U47123
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Brondyk WH, Groskreutz DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) R&D, Promega Corporation, 5445 East Cheryl 
            Parkway, Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Kenefick K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2000) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On May 9, 2000 this sequence version replaced U47123.1.
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Cloning vector pSP-luc+NF"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:45777"
     regulatory      join(1..3,4087..4103)
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase promoter"
     misc_feature    1
                     /label=SP6 RNA polymerase initiation site
                     /note="SP6 RNA polymerase initiation site"
     misc_feature    11..53
                     /label=upstream multiple cloning site
                     /note="upstream multiple cloning site"
     CDS             45..1700
                     /codon_start=1
                     /gene="luc+"
                     /product="luciferase"
                     /label=luc+
                     /note="firefly luciferase"
                     /protein_id="AAA88786.1"
                     /translation="MVTDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVA
                     PANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQ
                     SMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSH
                     ARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDY
                     KIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQG
                     YGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIM
                     SGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESI
                     LLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGV
                     VFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            45..1700
                     /gene="luc+"
                     /label=luc+
     CDS             54..1700
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="DAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHI
                     EVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPAN
                     DIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMY
                     TFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARD
                     PIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQ
                     SALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGL
                     TETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGY
                     VNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQ
                     HPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFV
                     DEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    1702..1731
                     /label=downstream multiple cloning site
                     /note="downstream multiple cloning site"
     regulatory      complement(1740..1759)
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        complement(1741..1759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1742
                     /label=T7 RNA polymerase initiation site
                     /note="T7 RNA polymerase initiation site"
     rep_origin      2014
                     /label=ColE1-derived replication origin
                     /note="ColE1-derived replication origin"
     rep_origin      complement(2017..2605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2776..3636)
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=ampR
                     /protein_id="AAA88787.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2776..3636)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(3637..3741)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4087..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"