LOCUS       Exported                3769 bp ds-DNA    circular SYN 17-8-2013
DEFINITION  Mammalian vector for the expression of secreted Lucia(R) luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSELECT-zeo-Lucia
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   InvivoGen
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     polyA_signal    9..57
                     /note="synthetic polyadenylation signal"
     misc_feature    68..159
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        178..688
                     /note="EF-1-alpha/HTLV composite promoter"
     CDS             728..1357
                     /codon_start=1
                     /product="secreted coelenterazine-utilizing luciferase"
                     /note="Lucia(R)"
                     /note="synthetic gene based on luciferases from marine 
                     copepods"
                     /translation="MEIKVLFALICIAVAEAKPTEINEDLNIAAVASNFATTDLETDLF
                     TNWETMNVISTDTEQVNTDADRGKLPGKKLPPDVLRELEANARRAGCTRGCLICLSHIK
                     CTPKMKKFIPGRCHTYEGEKESAQGGIGEAIVDIPEIPGFKDKEPLDQFIAQVDLCADC
                     TTGCLKGLANVQCSDLLKKWLPQRCTTFASKIQGRVDKIKGLAGDR"
     polyA_signal    1379..1500
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_site      1600..1994
                     /note="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2008..2382)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2392..2410)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2495..2698)
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2699..3002
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3082..3670)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"