LOCUS       Exported                4045 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector pRL-TK, complete sequence.
ACCESSION   AF025846
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Dual-Luciferase(TM) Reporter Assay: An Advanced Co-reporter 
            Technology
  JOURNAL   Promega Notes 57, 2-5 (1996)
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-1997) Production, Promega Co., 5445 East Cheryl 
            Parkway, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4045)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2000) Production, Promega Co., 5445 East Cheryl 
            Parkway, Madison, WI 53711, USA
REFERENCE   4  (bases 1 to 4045)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Feb 23, 2000 this sequence version replaced AF025846.1.
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Co-reporter vector pRL-TK"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:68406"
     regulatory      7..759
                     /regulatory_class="promoter"
                     /note="HSV TK promoter"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          826..962
                     /note="chimeric intron"
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1006..1024
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        1006..1024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1023
                     /label=T7 promoter transcription start site
                     /note="T7 promoter transcription start site"
     CDS             1034..1969
                     /codon_start=1
                     /gene="Rluc"
                     /product="Renilla luciferase"
                     /label=Rluc
                     /note="cDNA cloned from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /protein_id="AAB82579.1"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     gene            1034..1969
                     /gene="Rluc"
                     /label=Rluc
     polyA_signal    2000..2121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2011..2212
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
     promoter        2254..2358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2359..3219
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=ampR
                     /protein_id="AAB82580.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2359..3219
                     /gene="ampR"
                     /label=ampR
     rep_origin      3390..3978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"