LOCUS       Exported                3705 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector pRL-SV40, complete sequence.
ACCESSION   AF025845
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Dual-Luciferase(TM) Reporter Assay: An Advanced Co-reporter 
            Technology
  JOURNAL   Promega Notes 57, 2-5 (1996)
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-1997) Production, Promega Co., 5445 East Cheryl 
            Parkway, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 3705)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2000) Production, Promega Co., 5445 East Cheryl 
            Parkway, Madison, WI 53711, USA
REFERENCE   4  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Feb 18, 2000 this sequence version replaced AF025845.1.
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Co-reporter vector pRL-SV40"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:68405"
     regulatory      7..418
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          486..622
                     /note="chimeric intron"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      666..684
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    683
                     /label=T7 promoter transcription start site
                     /note="T7 promoter transcription start site"
     CDS             694..1629
                     /codon_start=1
                     /gene="Rluc"
                     /product="Renilla luciferase"
                     /label=Rluc
                     /note="cDNA cloned from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /protein_id="AAB82577.1"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     gene            694..1629
                     /gene="Rluc"
                     /label=Rluc
     polyA_signal    1660..1781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1671..1872
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
     promoter        1914..2018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2019..2879
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=ampR
                     /protein_id="AAB82578.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2019..2879
                     /gene="ampR"
                     /label=ampR
     rep_origin      3050..3638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"