LOCUS       Exported                4079 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector pRL-CMV, complete sequence.
ACCESSION   AF025843
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Dual-Luciferase(TM) Reporter Assay: An Advanced Co-reporter 
            Technology
  JOURNAL   Promega Notes 57, 2-5 (1996)
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Sherf B, Navarro S, Wood KV.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-1997) Production, Promega Co., 5445 East Cheryl 
            Parkway, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4079)
  AUTHORS   Grooms K.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-1999) Technical Writing, Promega Co., 2800 Woods 
            Hollow Rd., Madison, WI 53711, USA
REFERENCE   4  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Dec 17, 1999 this sequence version replaced AF025843.1.
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Co-reporter vector pRL-CMV"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:68403"
     regulatory      7..803
                     /regulatory_class="promoter"
                     /note="CMV enhancer and immediate-early promoter"
     enhancer        144..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          860..996
                     /note="chimeric intron"
     intron          863..995
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1040..1058
                     /regulatory_class="promoter"
                     /note="T7 promoter (17 to +2)"
     promoter        1040..1058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1057
                     /label=T7 promoter transcription start site
                     /note="T7 promoter transcription start site"
     CDS             1068..2003
                     /codon_start=1
                     /gene="Rluc"
                     /product="Renilla luciferase"
                     /label=Rluc
                     /note="cDNA cloned from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /protein_id="AAB82573.1"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     gene            1068..2003
                     /gene="Rluc"
                     /label=Rluc
     polyA_signal    2034..2155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2045..2246
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
     promoter        2288..2392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2393..3253
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=ampR
                     /protein_id="AAB82574.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2393..3253
                     /gene="ampR"
                     /label=ampR
     rep_origin      3424..4012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"