LOCUS       Exported                4351 bp ds-DNA    circular SYN 29-7-2013
DEFINITION  In vivo imaging vector for measuring promoter activity with 
            membrane-anchored Metridia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pMetLuc-Mem Reporter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    12..89
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             97..99
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             103..288
                     /codon_start=1
                     /product="signal-anchor region of the transferrin receptor"
                     /note="TfR membrane anchor"
                     /translation="VDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAV
                     IVFFLIGFMIGYLGYCK"
     CDS             322..927
                     /codon_start=1
                     /product="Metridia luciferase"
                     /note="MetLuc"
                     /note="human codon-optimized"
                     /translation="KSTEFDPNIDIVGLEGKFGITNLETDLFTIWETMEVMIKADIADT
                     DRASNFVATETDANRGKMPGKKLPLAVIMEMEANAFKAGCTRGCLICLSKIKCTAKMKV
                     YIPGRCHDYGGDKKTGQAGIVGAIVDIPEISGFKEMAPMEQFIAQVDRCASCTTGCLKG
                     LANVKCSELLKKWLPDRCASFADKIQKEVHNIKGMAGDR"
     polyA_signal    1051..1172
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1179..1634)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1661..1765
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        1856..2052
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      1903..2038
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2087..2881
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3113..3160
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      3489..4077
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4139..4187
                     /note="synthetic polyadenylation signal"
     misc_feature    4201..4292
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"