LOCUS       Exported                4079 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector phRL-CMV, complete sequence.
ACCESSION   AF362549
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     phRL-CMV Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Co-reporter vector phRL-CMV"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:159301"
     regulatory      7..803
                     /regulatory_class="promoter"
                     /note="CMV immediate early enhancer/promoter"
     enhancer        144..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          860..996
                     /note="chimeric intron"
     intron          863..995
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1040..1058
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        1040..1058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1057
                     /label=T7 promoter transcription start site
                     /note="T7 promoter transcription start site"
     regulatory      1062..1071
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1068..2003
                     /codon_start=1
                     /product="hRluc reporter"
                     /label=hRluc reporter
                     /note="humanized Renilla luciferase reporter"
                     /protein_id="AAK53374.1"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    2025..2246
                     /label=SV40 late poly(A) region
                     /note="SV40 late poly(A) region"
     polyA_signal    2034..2155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2288..2392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2393..3253
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance"
                     /protein_id="AAK53375.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3424..4012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"