LOCUS       Exported                4101 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector phRG-B, complete sequence.
ACCESSION   AF362550
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Zhuang Y, Kopps P, Kenefick KB.
  TITLE     phRG-B Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Zhuang Y, Kopps P, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4101)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Co-reporter vector phRG-B"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:159299"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      82..91
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             88..1023
                     /codon_start=1
                     /product="hRluc reporter"
                     /label=hRluc reporter
                     /note="humanized Renilla luciferase reporter"
                     /protein_id="AAK53376.1"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1055..1276
                     /label=SV40 late poly(A) region
                     /note="SV40 late poly(A) region"
     polyA_signal    1064..1185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1344..1363)
                     /label=RV primer 4
                     /note="RV primer 4"
     misc_feature    1601
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      complement(1604..2192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2363..3223)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance"
                     /protein_id="AAK53377.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3224..3328)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3355..3810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    3356..3810
                     /label=f1 origin
                     /note="f1 origin"
     misc_feature    3941..4094
                     /note="synthetic poly(A) signal/transcriptional pause 
                     region"
     polyA_signal    3941..3989
                     /note="synthetic polyadenylation signal"
     misc_feature    4003..4094
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4043..4062
                     /label=RV primer 3
                     /note="RV primer 3"