LOCUS       Exported                4818 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGL3-Basic, complete sequence.
ACCESSION   U47295
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Groskreutz DJ, Schenborn ET.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1996) D.J. Groskreutz, R&D, Promega Corporation, 
            5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Kenefick K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4818)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Mar 5, 2001 this sequence version replaced U47295.1.
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Cloning vector pGL3-Basic"
                     /mol_type="genomic DNA"
                     /label=luciferase reporter vector
                     /note="luciferase reporter vector"
                     /db_xref="taxon:45857"
     misc_feature    1..58
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      82..91
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             88..1740
                     /codon_start=1
                     /gene="luc+"
                     /product="luciferase"
                     /label=luc+
                     /protein_id="AAA89082.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            88..1740
                     /gene="luc+"
                     /label=luc+
     primer_bind     complement(89..111)
                     /label=GL primer2 sequencing primer binding site
                     /note="GL primer2 sequencing primer binding site"
     regulatory      1772..1993
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
     polyA_signal    1781..1902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2061..2080)
                     /label=RV primer4 sequencing primer binding site
                     /note="RV primer4 sequencing primer binding site"
     rep_origin      2318
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      complement(2321..2909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3080..3940)
                     /codon_start=1
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="ampicillin resistance"
                     /protein_id="AAA89083.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3080..3940)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(3941..4045)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4072..4527
                     /label=f1 origin
                     /note="f1 origin"
     regulatory      4658..4811
                     /regulatory_class="polyA_signal_sequence"
                     /note="upstream poly(A) signal"
     polyA_signal    4658..4706
                     /note="synthetic polyadenylation signal"
     misc_feature    4720..4811
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4760..4779
                     /label=RV primer3 sequencing primer binding site
                     /note="RV primer3 sequencing primer binding site"