LOCUS       Exported                5234 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Luciferase reporter vector pCBR-Control, complete sequence.
ACCESSION   AY258592
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Chroma-Luc? Series Reporter Vectors Technical Manual #TM061
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5234)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Luciferase reporter vector pCBR-Control"
                     /mol_type="other DNA"
                     /db_xref="taxon:236845"
     misc_feature    1..41
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      48..250
                     /regulatory_class="promoter"
                     /note="SV40 promoter"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      95..230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      274..283
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             280..1908
                     /codon_start=1
                     /product="CBRluc"
                     /label=CBRluc
                     /protein_id="AAP83305.1"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RYGTQLIPGVTVLVYLPFFHAFGFHITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSAIIQTLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGTEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLVKAGG"
     RBS             1230..1238
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    1942..2163
                     /label=SV40 late poly A
                     /note="SV40 late poly A"
     polyA_signal    1951..2072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2228..2419
                     /regulatory_class="enhancer"
     rep_origin      2734
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2737..3325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3496..4356)
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /note="AmpR"
                     /protein_id="AAP83304.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3496..4356)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(4357..4461)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4488..4943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    4489..4943
                     /label=f1 origin
                     /note="f1 origin"
     misc_feature    5074..5227
                     /label=upstream poly A
                     /note="upstream poly A"
     polyA_signal    5074..5122
                     /note="synthetic polyadenylation signal"
     misc_feature    5136..5227
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"