LOCUS       Exported                3538 bp ds-DNA    circular SYN 09-4-2014
DEFINITION  Vector for inducible high-level expression of C-terminally 
            V5-6xHis-tagged proteins in Drosophila cells. For other reading 
            frames, use pMT/V5-His B or pMT/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMT V5-His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
COMMENT     To create stable cell lines, co-transfect with the drug selection 
            vector pCoHygro or pCoBlast.
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        407..833
                     /note="MT promoter"
                     /note="Drosophila metallothionein promoter"
     misc_feature    849..929
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             930..971
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /note="V5 tag"
                     /translation="GKPIPNPLLGLDST"
     CDS             981..998
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     polyA_signal    1040..1174
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1720..2308)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2479..3339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3340..3444)
                     /gene="bla"
                     /note="AmpR promoter"