LOCUS       Exported                3596 bp ds-DNA    circular SYN 10-4-2014
DEFINITION  Insect cell vector for expression of secreted, C-terminally 
            V5-6xHis-tagged proteins. For other reading frames, use pMIB/V5-His 
            B or pMIB/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMIB V5-His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        1..548
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /note="OpIE-2 promoter"
                     /note="strong constitutive baculovirus promoter for insect 
                     cell expression"
     CDS             565..627
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /note="melittin signal sequence"
                     /translation="MKFLVNVALVFMVVYISYIYA"
     misc_feature    629..721
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             734..775
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /note="V5 tag"
                     /translation="GKPIPNPLLGLDST"
     CDS             785..802
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     polyA_signal    820..949
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /note="OpIE-2 poly(A) signal"
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1077..1665)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1740..2031
                     /gene="immediate-early gene 1 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /note="OpIE-1 promoter"
                     /note="moderate constitutive baculovirus promoter for 
                     insect cell expression"
     promoter        2057..2104
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             2123..2521
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="BSD"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2641..3501
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"