LOCUS       Exported                4821 bp ds-DNA    circular SYN 01-6-2015
DEFINITION  Baculovirus transfer vector for expression and secretion of 
            recombinant proteins. For other reading frames, use pMelBac A or 
            pMelBac C.
ACCESSION   .
VERSION     .
KEYWORDS    pMelBac B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
COMMENT     Exclusively for use with Bac-N-Blue(TM) DNA.
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        4..95
                     /gene="polh from Autographa californica"
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             96..158
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /note="melittin signal sequence"
                     /translation="MKFLVNVALVFMVVYISYIYA"
     misc_feature    158..213
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     225..1169
                     /note="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     CDS             complement(342..1169)
                     /codon_start=1
                     /product="baculovirus capsid-associated protein"
                     /note="ORF1629"
                     /note="required for viral replication"
                     /translation="FEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPS
                     EMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVA
                     MAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHN
                     ILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFY
                     KSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     promoter        1506..1610
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1611..2471
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2642..3230
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3407..4509)
                     /codon_start=3
                     /gene="lacZ (truncated)"
                     /product="N-terminal fragment of beta-galactosidase"
                     /note="5 lacZ fragment"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYTDRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQD"
     CDS             complement(4517..4606)
                     /codon_start=1
                     /gene="Autographa californica Ac-pcna"
                     /product="proliferating cell nuclear antigen (PCNA) from 
                     baculovirus"
                     /note="Ac PCNA"
                     /protein_id="
                     "
                     /translation="MHARLNKSSHRRRKISCIKEQLQLESLLKM"
     promoter        complement(4607..4821)
                     /note="ETL promoter"
                     /note="baculovirus early-to-late promoter (Liu et al., 
                     2006)"