LOCUS       Exported                3336 bp ds-DNA    circular SYN 09-4-2014
DEFINITION  Insect cell vector with a GFP-Zeocin(TM) resistance fusion gene, for
            constitutive expression of proteins with an optional C-terminal 
            V5-6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIZT V5-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        5..552
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /note="OpIE-2 promoter"
                     /note="strong constitutive baculovirus promoter for insect 
                     cell expression"
     misc_feature    567..656
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             663..704
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /note="V5 tag"
                     /translation="GKPIPNPLLGLDST"
     CDS             714..731
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     polyA_signal    749..878
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /note="OpIE-2 poly(A) signal"
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1669..1960
                     /gene="immediate-early gene 1 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /note="OpIE-1 promoter"
                     /note="moderate constitutive baculovirus promoter for 
                     insect cell expression"
     promoter        1986..2033
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             2052..2054
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             2067..2765
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996) lacking the 
                     unstructured C-terminal peptide"
                     /note="Cycle 3 GFP"
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHG"
     CDS             2766..3143
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
                     REFALRDPAGNCVHFVAEEQD"
     polyA_signal    3207..4
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"