pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.
| 载体基本信息 | |||
| 载体名称 | pIEx-4 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 3780 bp | 载体类型 | Insect Cell Vectors |
| 载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
| 启动子 | IE1 promoter | 克隆方法 | Enzyme Cut |
pIEx-4 载体载体图谱
pIEx-4 载体载体序列
LOCUS Exported 3780 bp ds-DNA circular SYN 26-4-2014
DEFINITION Vector for high-level expression in insect cells of proteins with a
C-terminal S-Tag-8xHis cassette.
ACCESSION .
VERSION .
KEYWORDS pIEx-4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3780)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3780
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
enhancer 1..483
/gene="Autographa californica hr5"
/note="hr5 enhancer"
/note="baculovirus early transcription enhancer"
promoter 487..1078
/note="IE1 promoter"
/note="promoter of the ie1 gene from the baculovirus
Autographa californica"
misc_feature 1082..1178
/note="MCS"
/note="multiple cloning site"
CDS 1084..1086
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 1189..1233
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/note="S-Tag"
/translation="KETAAAKFERQHMDS"
CDS 1255..1278
/codon_start=1
/product="8xHis affinity tag"
/note="8xHis"
/translation="HHHHHHHH"
terminator 1297..1604
/note="IE1 terminator"
/note="terminator of the ie1 gene from the baculovirus
Autographa californica"
promoter 1634..1738
/gene="bla"
/note="AmpR promoter"
CDS 1739..2599
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2770..3358
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
