LOCUS       Exported                4629 bp ds-DNA    circular SYN 26-4-2014
DEFINITION  Vector for high-level expression in insect cells of secreted 
            proteins with a cleavable N-terminal GST-6xHis-S-Tag cassette plus a
            C-terminal HSV tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     enhancer        1..483
                     /gene="Autographa californica hr5"
                     /note="hr5 enhancer"
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /note="IE1 promoter"
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             1084..1143
                     /codon_start=1
                     /product="signal sequence from tobacco hornworm 
                     adipokinetic hormone"
                     /note="AKH signal sequence"
                     /translation="MYKLTVFLMFIAFVIIAEAQ"
     CDS             1150..1806
                     /codon_start=1
                     /gene="
                     "
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /note="GST"
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             1834..1851
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             1861..1905
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /note="S-Tag"
                     /translation="KETAAAKFERQHMDS"
     CDS             1921..1938
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     CDS             1957..1971
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /note="enterokinase site"
                     /translation="DDDDK"
     misc_feature    1966..2080
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2086..2118
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /note="HSV tag"
                     /translation="QPELAPEDPED"
     terminator      2146..2453
                     /note="IE1 terminator"
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        2483..2587
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2588..3448
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3619..4207
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"