LOCUS       Exported                3801 bp ds-DNA    circular SYN 28-4-2014
DEFINITION  Vector for high-level expression in insect cells of secreted 
            proteins with a cleavable N-terminal Strep-Tag(R) II plus a 
            C-terminal 10xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     enhancer        1..483
                     /gene="Autographa californica hr5"
                     /note="hr5 enhancer"
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /note="IE1 promoter"
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             1084..1143
                     /codon_start=1
                     /product="signal sequence from mouse IgM heavy chain"
                     /note="IgM signal sequence"
                     /translation="MKFSWVMFFLMAVVTGVNSE"
     CDS             1156..1179
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /note="Strep-Tag II"
                     /translation="WSHPQFEK"
     CDS             1186..1200
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /note="enterokinase site"
                     /translation="DDDDK"
     misc_feature    1200..1259
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1270..1299
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /note="10xHis"
                     /translation="HHHHHHHHHH"
     terminator      1318..1625
                     /note="IE1 terminator"
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1655..1759
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1760..2620
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2791..3379
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"