LOCUS       Exported                2231 bp ds-DNA    circular SYN 21-4-2014
DEFINITION  Baculovirus transfer vector with a p10 promoter and a spectinomycin 
            resistance marker. Serves as a donor vector in the MultiBac(TM) 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pIDS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Geneva Biotech
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        16..125
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    133..196
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    261..309
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal"
     protein_bind    complement(511..544)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      546..902
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1365..2156
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"