LOCUS       Exported                2281 bp ds-DNA    circular SYN 21-4-2014
DEFINITION  Baculovirus transfer vector with a p10 promoter and a kanamycin 
            resistance marker. Serves as a donor vector in the MultiBac(TM) 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pIDK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Geneva Biotech
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        15..124
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    132..195
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    260..308
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal"
     protein_bind    complement(510..543)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      545..901
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1262..2056
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"