LOCUS       Exported                2312 bp ds-DNA    circular SYN 21-4-2014
DEFINITION  Baculovirus transfer vector with a polyhedrin promoter and a 
            chloramphenicol resistance marker. Serves as a donor vector in the 
            MultiBac(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pIDC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Geneva Biotech
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     protein_bind    complement(30..63)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      116..472
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(864..1523)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1524..1626)
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
     polyA_signal    1798..1932
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    2069..2154
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(2191..2282)
                     /gene="polh from Autographa californica"
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"