LOCUS       Exported                5379 bp ds-DNA    circular SYN 09-4-2014
DEFINITION  Vector for constitutive expression of C-terminally V5-6xHis-tagged 
            proteins in insect cells. For other reading frames, use 
            pAc5.1/V5-His A or pAc5.1/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pAc5.1 V5-His B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
COMMENT     To create stable cell lines, co-transfect with the drug selection 
            vector pCoHygro or pCoBlast.
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        56..2568
                     /note="Ac5 promoter"
                     /note="Drosophila melanogaster actin 5C promoter"
     misc_feature    2579..2663
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2664..2705
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /note="V5 tag"
                     /translation="GKPIPNPLLGLDST"
     CDS             2715..2732
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     polyA_signal    2805..2939
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3561..4149)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4320..5180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5181..5285)
                     /gene="bla"
                     /note="AmpR promoter"