| 载体基本信息 |
| 载体名称 |
pYX-Asc |
载体抗性 |
Ampicillin |
| 载体长度 |
1691 bp |
载体类型 |
I.M.A.G.E. Consortium Plasmids |
| 载体来源 |
I.M.A.G.E. Consortium |
拷贝数 |
High copy number |
pYX-Asc 载体载体图谱
pYX-Asc 载体载体序列
LOCUS Exported 1691 bp ds-DNA circular SYN 04-3-2013
DEFINITION Compact cDNA cloning vector.
ACCESSION .
VERSION .
KEYWORDS pYX-Asc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 1691)
AUTHORS I.M.A.G.E. Consortium
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..1691
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 4..22
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 40..99
/note="MCS"
/note="multiple cloning site"
promoter complement(137..155)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(217..805)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(814..1674)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
