LOCUS Exported 1804 bp ds-DNA circular SYN 09-11-2013
DEFINITION cDNA cloning vector derived from pOTB7.
ACCESSION .
VERSION .
KEYWORDS pOTB7a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 1804)
AUTHORS I.M.A.G.E. Consortium
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..1804
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 53..71
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
protein_bind 85..109
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB1"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind 111..127
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(282..300)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 404..992
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 1133..1792
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
LOCUS Exported 1804 bp ds-DNA circular SYN 09-11-2013
DEFINITION cDNA cloning vector derived from pOTB7.
ACCESSION .
VERSION .
KEYWORDS pOTB7a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 1804)
AUTHORS I.M.A.G.E. Consortium
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..1804
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 53..71
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
protein_bind 85..109
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB1"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind 111..127
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(282..300)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 404..992
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 1133..1792
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
