LOCUS       Exported                3392 bp ds-DNA    circular SYN 03-3-2013
DEFINITION  Mammalian vector for directional cloning and transient expression of
            a cDNA.
ACCESSION   AB009864
VERSION     .
KEYWORDS    pME18S-FL3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     A cDNA inserted between the DraIII sites can be excised with XhoI.
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     promoter        18..632
                     /note="SR-alpha promoter"
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     intron          662..755
                     /note="SV40 intron"
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    812..1194
                     /note="stuffer"
     polyA_signal    1297..1431
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1641..2229)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2400..3260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3261..3365)
                     /gene="bla"
                     /note="AmpR promoter"