LOCUS       Exported                4117 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pExpress-1, complete sequence.
ACCESSION   DQ845760
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Gruber CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2006) Express Genomics, Inc., 5 South Wisner 
            Street, Frederick, MD 21701, USA
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Cloning vector pExpress-1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:394265"
     polyA_signal    125..259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    136..498
                     /label=SV40 polyA
                     /note="SV40 polyA"
     misc_feature    637..946
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     664..680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(772..790)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(801..817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      812..1334
                     /regulatory_class="promoter"
                     /note="CMV"
     promoter        complement(943..1146)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1147..1450
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      1765..2404
                     /label=pUC
                     /note="pUC"
     rep_origin      complement(1910..2498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2669..3529)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="Amp"
                     /protein_id="ABG74348.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3530..3634)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    3545..4001
                     /label=f1
                     /note="f1"
     rep_origin      complement(3661..4116)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"