LOCUS       Exported                4161 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDNR-LIB, complete sequence.
ACCESSION   DQ666274
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Kalnine N, Farmer A, Hsieh F, Zhang R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella 
            ave., Mountain View, CA 94043, USA
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Cloning vector pDNR-LIB"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:390513"
     misc_feature    9..42
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    9..42
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    45..99
                     /note="MCS A; multiple cloning site"
     misc_feature    100..291
                     /label=stuffer fragment
                     /note="stuffer fragment"
     polyA_signal    162..296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    292..351
                     /note="MCS B; multiple cloning site"
     terminator      415..509
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(530..1189)
                     /codon_start=1
                     /gene="CmR"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /protein_id="ABG24168.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(530..1189)
                     /gene="CmR"
                     /label=CmR
     misc_feature    1218..1251
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    1218..1251
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    complement(1266..3168)
                     /label=SacB negative selection marker
                     /note="SacB negative selection marker"
     CDS             complement(1302..2723)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(2724..3169)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      3337..3980
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     rep_origin      3360..3948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4065..4081
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4089..4107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4091..4109
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase"