LOCUS       Exported                4938 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDNR-Dual, complete sequence.
ACCESSION   DQ666273
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Kalnine N, Farmer A, Lin Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella 
            ave., Mountain View, CA 94043, USA
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Cloning vector pDNR-Dual"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:390512"
     regulatory      9..42
                     /regulatory_class="other"
                     /note="loxP recombination site"
     protein_bind    9..42
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    45..129
                     /note="MCS; multiple cloning site"
     misc_feature    132..136
                     /label=splice donor (SD) site
                     /note="splice donor (SD) site"
     misc_feature    141..176
                     /label=6xHN epitope tag
                     /note="6xHN epitope tag"
     CDS             141..176
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
     terminator      212..306
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(327..986)
                     /codon_start=1
                     /gene="CmR"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /protein_id="ABG24167.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(327..986)
                     /gene="CmR"
                     /label=CmR
     misc_feature    1015..1048
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    1015..1048
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1057..2972
                     /label=SacB negative selection marker
                     /note="SacB negative selection marker"
     promoter        1063..1508
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             1509..2930
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        2998..3102
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3103..3963
                     /codon_start=1
                     /gene="Amp"
                     /product="ampicillin resistance protein"
                     /label=Amp
                     /protein_id="ABG24166.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3103..3963
                     /gene="Amp"
                     /label=Amp
     rep_origin      4111..4574
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     rep_origin      4134..4722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4839..4855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4863..4881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"