LOCUS       Exported                4707 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Monster GFP vector phMGFP, complete sequence.
ACCESSION   AY218848
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Sundquist T, Chauvin F, Almond B.
  TITLE     Monster Green Fluorescent Protein phMGFP Vector Technical Bulletin, 
            TB320
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Sundquist T, Chauvin F, Almond B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4707)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Monster GFP vector phMGFP"
                     /mol_type="genomic DNA"
                     /note="green fluorescent protein cloned from Montastraea 
                     cavernosa, the great star coral"
                     /db_xref="taxon:220994"
     regulatory      1..742
                     /regulatory_class="promoter"
                     /note="CMV enhancer/promoter"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          857..989
                     /note="chimeric intron"
     regulatory      1034..1052
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1070..1079
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1076..1759
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /note="hMGFP"
                     /protein_id="AAO43180.1"
                     /translation="MGVIKPDMKIKLRMEGAVNGHKFVIEGDGKGKPFEGKQTMDLTVI
                     EGAPLPFAYDILTTVFDYGNRVFAKYPKDIPDYFKQTFPEGYSWERSMTYEDQGICIAT
                     NDITMMKGVDDCFVYKIRFDGVNFPANGPVMQRKTLKWEPSTEKMYVRDGVLKGDVNMA
                     LLLEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHRIEIVSHDKDYNKVKLYEHAEAHSGL
                     PRQAG"
     promoter        complement(1781..1798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1807..2028
                     /label=SV40 late polyadenylation signal
                     /note="SV40 late polyadenylation signal"
     polyA_signal    1816..1937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2123..2578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2910..3014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3015..3875
                     /codon_start=1
                     /product="B-lactamase"
                     /label=B-lactamase
                     /note="AmpR"
                     /protein_id="AAO43181.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4046..4634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"