LOCUS       Exported               11342 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  contains the fully codon optimized ORF1 and ORF2 of the human L1RP .
ACCESSION   .
VERSION     .
KEYWORDS    pBS-L1PA1-CH-mneo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Wagstaff BJ, Barnerssoi M, Roy-Engel AM
  TITLE     Evolutionary conservation of the functional modularity of primate 
            and murine LINE-1 elements.
  JOURNAL   PLoS One. 2011 May 10;6(5):e19672. doi: 
            10.1371/journal.pone.0019672.
  PUBMED    21572950
REFERENCE   2  (bases 1 to 11342)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-05-10"; volume: "6(5):e19672. doi"; pages: " 
            10.1371/journal.pone.0019672"
FEATURES             Location/Qualifiers
     source          1..11342
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        935..1314
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1315..1518
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1469..1489
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1515..1539
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(6655..6674)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8166..8185
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8295..8624)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8309..8444
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8363..8382)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8609..8629
                     /label=pBABE 3
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    8729..8863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8753..8772)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8807..8826
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(9154..9174)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9154..9172)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9193..9209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9193..9209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9206..9228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9217..9233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9241..9271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9286..9307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9424..9441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9595..10183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9675..9694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10354..11214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10977..10996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11215..11319)
                     /gene="bla"
                     /label=AmpR promoter