LOCUS       Exported                4474 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from T to
            A at position 308 and S to A at position 473 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    pBSFI-myr-akt-T308A/S473A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             63..83
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1536..1562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1631..1650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1632..1650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1657..1674)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1657..1673)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1666..1688)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1814..2269)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1951..1972)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2163..2182
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2296..2400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2401..3261
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2619..2638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3432..4020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3921..3940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4174..4191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4308..4329
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4344..4374
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4382..4398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4387..4409
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4406..4422
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4406..4422
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4443..4461
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"