This plasmid contains the coding sequence of a dimeric vesion of the fluorescent protein “Keima-Red,” which was cloned from the stony coral whose Japanese name is“Komon-Sango.” CoralHue dKeima-Red absorbs light maximally at 440 nm and emits red light at 616 nm. Thus CoralHue dKeima-Red exhibits an extremely large Stokes shift (176 nm). The red fluorescence is stable under usual aerobic conditions. The combination of red emission, a very large Stokes shift, stability at 37℃ in eukaryotic cells, and being dimeric make dKeima-Red a superb reporter protein for labeling proteins or subcellular structures in multicolor fluorescence analyses.
| 载体基本信息 | |||
| 载体名称 | pdKeima-Red-S1 (Dimeric Keima-Red) | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 3348 bp | 载体类型 | FRET assay vectors |
| 5引物 | M13 fwd | 3引物 | M13 rev |
pdKeima-Red-S1 (Dimeric Keima-Red) 载体载体图谱
pdKeima-Red-S1 (Dimeric Keima-Red) 载体载体序列
LOCUS Exported 3348 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3348)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3348
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 96..200
/gene="bla"
/note="AmpR promoter"
CDS 201..1061
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1232..1820
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2108..2129
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2144..2174
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2182..2198
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2206..2222
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 2264..2932
/codon_start=1
/product="dimeric Keima-Red fluorescent protein"
/note="dKeima-Red"
/translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
TKGGPLPFAWDILSPLFQYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTV
SNDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
LEGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
"
primer_bind complement(2954..2970)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
