| 载体基本信息 |
| 载体名称 |
pTE-10M0X |
载体抗性 |
Hygromycin |
| 载体长度 |
6103 bp |
载体类型 |
Bacterial Expression |
| 拷贝数 |
Low Copy |
克隆方法 |
Restriction Enzyme |
pTE-10M0X 载体载体图谱
pTE-10M0X 载体载体序列
LOCUS Exported 6103 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTE-10M0X
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6103)
AUTHORS Klotzsche M, Ehrt S, Schnappinger D
TITLE Improved tetracycline repressors for gene silencing in mycobacteria.
JOURNAL Nucleic Acids Res. 2009 Jan 27. ():.
PUBMED 19174563
REFERENCE 2 (bases 1 to 6103)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. 2009 Jan 27. ():."
FEATURES Location/Qualifiers
source 1..6103
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 38..54
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 39..55
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 1056..1083
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(1173..1190)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1344..1932)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1424..1443)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
