LOCUS       Exported                8897 bp ds-DNA    circular SYN 14-1-2016
DEFINITION  P element-based vector for Gal4-regulated expression of genes in 
            Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    pUAST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Brand AH, Perrimon N.
  TITLE     Targeted gene expression as a means of altering cell fates and 
            generating dominant phenotypes.
  JOURNAL   Development 1993;118:401-15.
  PUBMED    8223268
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Brand Lab
  TITLE     Direct Submission
COMMENT     http://www.gurdon.cam.ac.uk/~brandlab/3.html
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..46
                     /note="MCS"
                     /note="multiple cloning site"
     intron          124..189
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     polyA_signal    611..745
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             join(1250..1321,1716..1989,2064..2718,2780..3095,3316..3447,
                     3518..4132)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /note="mini-white"
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /protein_id="
                     "
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNVSSTCAYILSSLDL*ELRLQH
                     QIVCGVRTATHKNPKPQSHKQIVTRNRLFW*LCSLYKTIFKIIS*SRHLKAFIFDYILF
                     YKKYNNQIF*ADPRCTKNK*KYKPTS*DTSFCSGLDEHNACS*YLRSPIIAG*QRSGFA
                     ELH*PGLRAGQKLRHAPATQSAGGLRFRERPTSREPHLCLAQYGHLWGGQSAGLRMAAA
                     GQPDTRTILQRATHTGAQETFAQER*VSIRSRLICVKS**RVQLPI*NSVCGVAYPGEL
                     LAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNGQPVDAKEMQARCAYVQQDDLF
                     IGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQELSLSKCQHTIIGVPGRVKGLSG
                     GERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQVLKKLSQKGKTVILTIHQPSS
                     ELFELFDKILLMAEGRVAFLGTPSEAVDFFS**VRCVY*GYLALHYISTPIQRGCPVSY
                     QLQSGGLLRTGVGRCARTGDRVP*SDRQDMRQFCY*QSSPGYGAVVGHQKFGEATGAAG
                     EWVHLQGHLVHAVPGGPVAILAVGAQGTTPRKSATYSDNGEWFQWKQMI*RLQFLETNS
                     LDFS*NCLIPHPS*FFSMRCIVYSFAENK*ISFNSRTC*RYEY**DASNILICRWLPS*
                     LASSFWANNSRKWA**ISTEPSSSS*PT*PFKTSLPR*M*VLFRIHLHINNLLTF**ID
                     SI*VFTSELPVFMREARSRLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGV
                     LHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVP
                     VYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADL
                     PLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    4910..5495
                     /note="P element 5 end"
     misc_feature    5496..5724
                     /note="white linker sequence"
     rep_origin      complement(5730..6318)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6489..7349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7350..7454)
                     /gene="bla"
                     /note="AmpR promoter"
     misc_feature    7755..8255
                     /note="white linker sequence"
     misc_feature    8256..8488
                     /note="P element 3 end"
     protein_bind    8533..8627
                     /bound_moiety="GAL4"
                     /note="5X UAS"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        8646..8884
                     /note="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"