LOCUS       Exported                2462 bp ds-DNA    circular SYN 24-6-2015
DEFINITION  Cloning vector for in vitro transcription using the SP6 and T7 RNA 
            polymerase promoters. Essentially identical to pSP73 except for the 
            orientation of the MCS.
ACCESSION   X65332
VERSION     .
KEYWORDS    pSP72
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Krieg PA, Melton DA.
  TITLE     In vitro RNA synthesis with SP6 RNA polymerase.
  JOURNAL   Meth. Enzymol. 1987;155:397-415.
  PUBMED    2828872
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Promega
  TITLE     Direct Submission
COMMENT     
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    3..90
                     /gene="
                     "
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(100..118)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(376..964)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1135..1995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1996..2100)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        2446..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"